Helicobacter pylori antigen rapid test Rapid diagnosis of Helicobacter pylori antigen test
-
$0.30 / 1000 - 4999 pieces
$0.25 / 5000 - 9999 pieces
$0.20 / >=10000 pieces - 1000 pieces
- Biocare Diagnostics Ltd.
- Guangdong, China
- Mr
Product Detail
| Shelf life: | 24 months | port: | Zhuhai |
| Package Size: | 25 tests/kit, 10 tests/kit | Certificate: | ISO13485 |
| Supply capacity: | 1000 pieces per day | Storage: | 4-30℃ |
| product name: | Helicobacter pylori antigen test | brand: | Biocare or OEM,Biocare |
| specimen: | human feces | payment terms: | L/C, Western Union, D/P, D/A, T/T, MoneyGram |
| place of origin; place of origin: | Guangdong, China |
Specification
brand | biological care |
product name | Helicobacter pylori antigen test |
Package Size | 25 tests/kit, 10 tests/kit |
specimen | human feces |
brand | Biocare or OEM |
shelf life | 24 months |
Certificate | ISO13485 |
storage | 4-30℃ |
Product Description
possible use
The Helicobacter pylori antigen test is an in vitro qualitative immunochromatographic method for the rapid detection of Helicobacter pylori antigens in human stool specimens. The test results are intended to help diagnose H. pylori infection, monitor the effectiveness of treatment and confirm the eradication of H. pylori in peptic ulcer patients.
The Helicobacter pylori antigen test is an in vitro qualitative immunochromatographic method for the rapid detection of Helicobacter pylori antigens in human stool specimens. The test results are intended to help diagnose H. pylori infection, monitor the effectiveness of treatment and confirm the eradication of H. pylori in peptic ulcer patients.
Test Principle
The Helicobacter pylori antigen test is a sandwich solid-phase immunochromatographic test. To perform the test, an aliquot of the diluted stool sample is added to the sample well of the test cartridge. The sample flows through a marker pad containing an antibody to Helicobacter pylori conjugated to red colloidal gold. If the sample contains Helicobacter pylori antigen, the antigen will combine with the antibody coated on the colloidal gold particles to form an antigen-antibody-gold complex. These complexes move by capillary action on the nitrocellulose membrane towards the area of the test line where the H. pylori-specific antibody is immobilized. When the complexes reach the test line, they will bind to the antibody on the membrane in the form of a line. A second red control line will always appear in the results window, indicating that the test was performed correctly and that the test equipment is functioning properly. If the H. pylori antigen is absent or below the detection limit of the test, only the control line will be seen. If the control line does not develop, the test is invalid.
The Helicobacter pylori antigen test is a sandwich solid-phase immunochromatographic test. To perform the test, an aliquot of the diluted stool sample is added to the sample well of the test cartridge. The sample flows through a marker pad containing an antibody to Helicobacter pylori conjugated to red colloidal gold. If the sample contains Helicobacter pylori antigen, the antigen will combine with the antibody coated on the colloidal gold particles to form an antigen-antibody-gold complex. These complexes move by capillary action on the nitrocellulose membrane towards the area of the test line where the H. pylori-specific antibody is immobilized. When the complexes reach the test line, they will bind to the antibody on the membrane in the form of a line. A second red control line will always appear in the results window, indicating that the test was performed correctly and that the test equipment is functioning properly. If the H. pylori antigen is absent or below the detection limit of the test, only the control line will be seen. If the control line does not develop, the test is invalid.
User Notes
1. For in vitro diagnostic use only.
2. Do not use kits that have expired.
3. Do not mix components from kits of different lot numbers.
4. Avoid reagent contamination by microorganisms.
5. Do not suck the reagent with your mouth, do not smoke or eat during the test.
6. Wear gloves throughout the process to avoid splashing of reagents or specimens.
7. Wipe up spills with 5% hypochlorite solution.
8. Decontaminate all liquid or solid waste prior to disposal.
1. For in vitro diagnostic use only.
2. Do not use kits that have expired.
3. Do not mix components from kits of different lot numbers.
4. Avoid reagent contamination by microorganisms.
5. Do not suck the reagent with your mouth, do not smoke or eat during the test.
6. Wear gloves throughout the process to avoid splashing of reagents or specimens.
7. Wipe up spills with 5% hypochlorite solution.
8. Decontaminate all liquid or solid waste prior to disposal.
specimen collection
Fecal specimens should be collected in containers that do not contain media, preservatives, animal serum, or detergents, as any of these additives may interfere with the HP Ag test. Specimens can be stored at 2-8°C for 3 days without affecting assay performance. For long-term storage of samples, -20°C or lower is recommended. Repeated freezing and thawing of specimens is not recommended and may lead to erroneous results. Do not store specimens in self-defrosting refrigerators.
Storage of test suites
HP Ag Test can be stored at any temperature between 4-30°C. Do not freeze. The stability of the kit under these storage conditions is 24 months. Use up the reagents as soon as possible within 3 months after the kit is opened.
assay procedure
1. Allow all reagents to come to room temperature before use.
2. Place 0.5-1ml of extraction buffer into an appropriately labeled tube.
3. Add approximately 5-6 mm sized (25-100 mg) sample portions with cotton swabs, wooden applicators or bacteriological loops. Press the applicator onto the tube and swirl at the same time. For liquid or semi-solid stools, add 100 µl of stool using a suitable pipette.
4. Vortex or stir to release the virus into the dilution.
5. Add 3-4 drops to the sample well of the test device.
6. Incubate the strip at room temperature and read the strip after 5-15 minutes.
Interpretation of results
Fecal specimens should be collected in containers that do not contain media, preservatives, animal serum, or detergents, as any of these additives may interfere with the HP Ag test. Specimens can be stored at 2-8°C for 3 days without affecting assay performance. For long-term storage of samples, -20°C or lower is recommended. Repeated freezing and thawing of specimens is not recommended and may lead to erroneous results. Do not store specimens in self-defrosting refrigerators.
Storage of test suites
HP Ag Test can be stored at any temperature between 4-30°C. Do not freeze. The stability of the kit under these storage conditions is 24 months. Use up the reagents as soon as possible within 3 months after the kit is opened.
assay procedure
1. Allow all reagents to come to room temperature before use.
2. Place 0.5-1ml of extraction buffer into an appropriately labeled tube.
3. Add approximately 5-6 mm sized (25-100 mg) sample portions with cotton swabs, wooden applicators or bacteriological loops. Press the applicator onto the tube and swirl at the same time. For liquid or semi-solid stools, add 100 µl of stool using a suitable pipette.
4. Vortex or stir to release the virus into the dilution.
5. Add 3-4 drops to the sample well of the test device.
6. Incubate the strip at room temperature and read the strip after 5-15 minutes.
Interpretation of results
Interpretation of results
Negative: A pink line appears on the control line, indicating a correct test.
Negative: A pink line appears on the control line, indicating a correct test.
There will be no lines in the testing area.
Positive: A distinct pink band appears in the test area in addition to the pink control line.
Invalid: The complete absence of color in both areas indicates a procedural error and/or test reagents have
deterioration. The test should be repeated with new test strips.
Positive: A distinct pink band appears in the test area in addition to the pink control line.
Invalid: The complete absence of color in both areas indicates a procedural error and/or test reagents have
deterioration. The test should be repeated with new test strips.
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