Solarbio High Quality Soil Nitrite Reductase (S-NiR) Assay Kit
- 1 piece
- Beijing Solarbio Science & Technology Co., Ltd.
- Beijing, China
- Ms Wong Amber
Product Detail
Soil nitrite reductase (S-NiR) detection kit
| notes: | Take two or three different samples for prediction before testing. |
| Operating equipment: | Spectrophotometer |
| Catalog number: | BC2990 |
| size: | 50T/24S |
Element:
Reagent 1: Powder × 1, store at 4°C. Dissolve in 1 mL of distilled water before use. Reagents can be stored at 4°C for 2 weeks. Dilute 400 times with distilled water before use.
Reagent 2: Powder × 1, store at 4°C. Dissolve in 15mL distilled water before use. Reagents can be stored at 4°C for 2 weeks.
Reagent 3: 15mL×1, store at 4°C. This solution is a saturated solution, just take the supernatant
Reagent 4: 25mL×1, store at room temperature away from light.
Reagent 5: 25mL×1, store at room temperature away from light.
Standard: 1 mL×1, stored at 4°C. 10 μmol/mL NaNO2Standard solution.
Soil nitrite reductase (S-NiR) is one of the key enzymes in denitrification. It is a reductase produced by soil denitrifying bacteria. It can reduce NO2-to NO. This activity reflects the conversion efficiency of nitrogen during the biodegradation process and provides a certain basis for the study of nitrogen conversion.
Nitrite reductase reduces NO2-to NO, and reduce NO2-Nitrite in the sample participates in the diazotization reaction to generate a purple-red compound, that is, the change in absorbance at 540nm can reflect the activity of nitrite reductase in the soil.
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