Custom Rapid Delivery Influenza A/B Rapid Cassette Test

【Possible use】The Influenza A/B Rapid Cassette Test is an in vitro qualitative immunochromatographic assay for the rapid detection of Influenza A and B from nasal swabs, nasopharyngeal washes, or aspiration specimens. The Influenza A/B Rapid Cassette Test provides a simple and highly sensitive screening method for the presumptive diagnosis of influenza A and/or influenza B respiratory infection.【Summary】Influenza is caused by a virus that primarily attacks the upper airways -- the nose, throat and bronchi, and rarely the lungs. The infection usually lasts for about a week. It is characterized by sudden onset of high fever, myalgia, headache and severe malaise, dry cough, sore throat and rhinitis. Most people recover within one to two weeks without any treatment. Influenza poses a serious risk for the very young, the elderly and people with medical conditions such as lung disease, diabetes, cancer, kidney or heart problems. In these individuals, infection can lead to serious complications of the underlying disease, pneumonia, and death. Currently circulating influenza viruses that cause human disease are divided into two types, A and B. Influenza A has 3 subtypes that are important to humans: A(H3N2), A(H1N1) and A(H5N1), with the former being currently associated with most deaths. Influenza viruses are defined by 2 different protein components called antigens on the surface of the virus. They are spike-like features called hemagglutinin (H) and neuraminidase (N) components. Influenza A and B rapid cassette detection is suitable for clinical systems, health care institutions, scientific research and other fields. It is only used for clinical auxiliary diagnosis of influenza A/B virus infection, and cannot be used alone as a basis for confirming or excluding cases.【Test principle】The kit adopts colloidal gold immunochromatography technology and double-antibody sandwich method to detect influenza A and B antigens in human nasopharyngeal or oropharyngeal swab samples. The test device consists of two test papers based on the same technical principle. They are placed side by side. When testing, the same sample is added to two sample pads to achieve the purpose of joint testing. Take influenza A test paper as an example to illustrate its detection principle: the binding pad on the test paper is pre-coated with colloidal gold-labeled murine influenza A monoclonal antibody, that is, the gold-labeled detection antibody; the detection line on the nitrocellulose (NC) membrane ( Line T) and quality control line (line C) are pre-coated Flu A capture antibody and goat anti-mouse IgG polyclonal antibody, respectively. When a positive sample is detected, the influenza A antigen in the sample combines with the gold-labeled antibody to form a complex. Under the action of chromatography, the complex moves forward along the test strip and is captured by the T-line capture antibody, forming a purple-red line visible to the naked eye; when testing negative samples, there is no visible line in the T-line area. Under normal detection conditions, whether it is a negative sample or a positive sample, the uncomplexed gold-labeled antibody and the uncaptured complex continue to move forward and are captured by the goat anti-mouse IgG polyclonal antibody of C line area, C line The area will form a magenta line, indicating that the test is valid.

【Storage and shelf life】The Influenza A/B Rapid Cassette Test Kit can be stored at any temperature between 4-30°C. Do not freeze. The stability of the kit under these storage conditions is 24 months. Use up the reagents as soon as possible within 3 months after the kit is opened.【Specimen Collection】1. Sample extraction solution: Avoid contamination every time the sample extraction solution is taken, so as not to breed bacteria and make it unusable next time. 2. Collection of nasal secretions: The operator inserts a sterile swab into the place with the most nasal secretions, puts the swab against the inner wall of the nasal cavity and rotates it 3 times, and then takes out the swab. 3. Throat secretion collection: The operator fully inserts a sterile swab into the throat from the mouth, centering on the reddened part of the throat wall, palate, and tonsils, wipes it with moderate force and rotates it 3 times, avoiding touching the tongue, and takes out the swab. 4. The sample should be used as soon as possible after collection, otherwise it should be sealed and stored in a container at 2-8⁰C refrigerator, but not more than 24 hours.【testing method】1. Add 15 drops (1) of Reagent B and immediately place the swab into the test tube. 2. Mix the solution by swirling the swab vigorously against the tube wall for at least 1 minute. Best results are obtained when the sample is vigorously extracted in solution (2). Aspirate as much fluid as possible from the swab by squeezing or swirling the swab against the side of the tube as you withdraw the swab. Discard the swab. 3. Remove the Influenza A/B Rapid Kit from the sealed bag before use. 4. Use a separate pipette for each sample or control. From the tube put exactly 4 drops into the circular window marked letter A (3) and from the same tube place 4 drops into the circular window marked letter B (4). 5. Read the result after 10 minutes. Do not read test results after 10 minutes. If the test was not run due to the sample type, pipette to stir the sample added in the sample window. if it

【Interpretation of results】 Negative:The test card only has a red line on the C line.Influenza A positive:Two red lines appear on the T line and C line of A.

(This picture is for operational reference only, and the actual product shall prevail.) Note: The red line on the T line may have different shades of color, which is due to the different levels of influenza A/B antigens in samples. Even a weaker color line should be judged as a positive result within the specified observation time, no matter how dark the color line is.