High quality SYBR Green II RNA for Scientific Research

SYBR Green II RNA Gel Stain is one of the most sensitive dyes known for detecting RNA in electrophoretic gels. Using 254 nm epi-illumination Polaroid® 667, black and white print film, and SYBR Green gel-stained photographs, as little as 100 pg RNA or ssDNA per band can be detected in SYBR Green II-stained agarose or polyacrylamide gels for screening. Even with 300 nm transillumination, as little as 500 pg of RNA per band can be detected. SYBR Green II RNA Gel Stain is more sensitive than ethidium bromide, the most commonly used stain for detecting nucleic acids in gels (Figure 1). The sensitivity limit of ethidium bromide in standard agarose minigels is approximately 1.5 ng single-stranded nucleic acid per band by 300 nm transillumination and photography through an orange-red gelatin filter. Please note that our detection limits are based on results obtained using a FotoDyne® Foto/UV® 450 UV Transilluminator with Polaroid 667 Film. Video cameras and CCD cameras generally have a different spectral response than black and white printed film and therefore may not exhibit the same sensitivity.

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